Leica SP5 II Laser Scanning Confocal Microscope with HyD upgrade
The SP5 II offers true confocal scanning and is equipped with 6 detectors (2 HyD, 4 PMT including transmitted light) across 400-800 nm (1 nm step) and 8 laser excitation lines (405, 458, 476, 488, 496, 514, 594 and 633 nm). Excitation-emission light separation is achieved by an Acoustic Optical Beam Splitter. The resonant laser scanner has a maximum line frequency of 16 kHz. In addition, detection of transmitted light for BF, DIC and Pol channels is possible.
Scan modes available include xyzt and λ and a motorised stage permits tile scans and mark and find experiments, with additional licenses for colocalization, FRET and FRAP. The SP5 II is supported by the Huygens deconvolution package from SVI.
A live cell imaging capability includes a chamber to maintain temperature and supply premixed gasses, as is appropriate for many mammalian tissue culture systems. Conventional tissue culture incubators are co-located with the microscopes to facilitate experiments.